Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-DcR2 Antibody, intracellular domain detects level of DcR2 & has been published & validated for use in WB, IH.

Western blot: 1:100 to 1:4000

Immunohistochemistry: 10 µg/mL


Whole cell lysate from HeLa cells can be used as a positive control .


Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: intracellular domain

Polyclonal antibody raised against a peptide corresponding to amino acids 249-263 of human DcR2 precursor (Marsters et al. 1997; Degli-Esposti et al. 1997; Pan et al. 1997).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Specificity

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a decoy receptor (Pan et al. 1997a,b; Sheridan et al. 1997). Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL-R4, or TRUNDD (Marsters et al. 1997; Degli-Esposti et al. 1997; Pan et al. 1997). DcR2 has an extracellular TRAIL binding domain but lacks intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuated TRAIL-induced apoptosis.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.